Rest 2005
نویسنده
چکیده
3 ©2005 Corbett Research Pty Ltd and Michael W. Pfaffl 1 Abstract REST 2005 is a new standalone software tool to estimate up and down-regulation for gene expression studies. The software addresses issues surrounding the measurement of uncertainty for expression ratios, by using randomisation and bootstrapping techniques. By increasing the number of iterations from 2,000 to 50,000 in this version hypothesis tests achieve a level of consistency on par with traditional statistical tests. New confidence intervals for expression levels also allow scientists to measure not only the statistical significance of deviations, but also their likely magnitude, even in the presence of outliers. Graphical output of the data via a whisker box-plots provide a visual representation of variation for each gene that highlights potential issues such as distribution skew.
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